DNA Melting - Temperature Profile

DNA sequence input

How to input a sequence

Upload a sequence file:

The sequence's GI number: in the NCBI GenBank. NCBI's Disclaimer and Copyright
Insert DNA sequence (copy/paste):

Extract DNA sequence fragment (optional):

Range: to Default [begin-end] to get the full sequence Default [begin-end] to get the full sequence

Sequence length limitation: 20000bp

Choose probability level for the temperature profile

How to handle the temperature profile

Plot Tp(i) for p=

When using p=0.5 (the default), the resulting plot is a "Tm profile".

Advanced options

What is this

Choose Loop Entropy Factor: Multiexponential approximation (fast) Exact power law (slow)

Choose empiric thermodynamic parameter set:

Salt concentration: [Na+] = M (Interval: [0.001 - 8])

This might take up 2-3min to analyze.
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